Journal: Blood Advances
Article Title: HCAR2 is a novel receptor for heme ∗
doi: 10.1182/bloodadvances.2025016197
Figure Lengend Snippet: Biochemical validation of HCAR2 as a receptor for heme, illustrated by direct ligand/receptor binding. (A) Comparison by SPR of the interaction of immobilized recombinant HCAR2 compared to C3b (negative control) with different concentrations of heme (39 to 2500 nM). (B) Analysis of HCAR2-heme interaction by spectroscopy (0.35 to 10.2 μM). (C) Absorbance at the Soret peak (414 nM) as a function of the molar excess of heme over the protein. RU, response units.
Article Snippet: Recombinant HCAR2 (NM-177551; Origene) and C3b (Comp Tech A114; serving as a negative control, because it does not bind heme) were immobilized on the general long chain sensor chips of Biacore 2000 equipment, following the manufacturer’s instructions.
Techniques: Biomarker Discovery, Binding Assay, Comparison, Recombinant, Negative Control, Spectroscopy